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ID AN00181569-19871200-1001
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タイトル Synthesis of the Disaccharides of Phenolic Glycolipid I of Mycobacterium leprae with Some Kinds of Aglycone and Their Serological Activities
著者
藤原 剛 (Fujiwara tsuyoshi)
教養部
和泉 真蔵 (izumi shinzo)
教養部
publisher
出版地 奈良
出版者 奈良大学
上位タイトル
奈良大学紀要 (Memoirs of the Nara University). Vol.16号, (1987. 12) ,p.1- 10
識別番号
ISSN
03892204
抄録 The bromide of the peracetate of the non-reducing-end disaccharide of phenolic glycolipid I of Mycobacterium leprae was coupled with lauryl alcohol or 3-phenylpropanol in acetonitrile in the presence of mercury cyanide. The product was the 1:1 mixture of α-and β-1inked disaccharides. The coupling of the bromide with stearyl alcohol in nitromethane-toluene gave mainly α―linked stearyl disaccharide. The complete loss of the stereoselectivity in acetonitrile was thought to be due to the lack of neibouring group participation.
The activities of these disaccharides and some other synthetic disaccharides
were tested in ELISA inhibition assay and direct ELISA. No compound tested in this study showed lower activity than free disaccharide in ELISA inhibition assay. Among the tested disaccharides, the compounds with long lipophilic chain as lauryl and stearyl disaccharides showed high activity in both ELISA inhibition assay and direct ELISA. On the other hand the compounds with short chain showed only the same level of the activity as that of free disaccharide in ELISA inhibition assay. And these compounds showed no activity in direct ELISA. These results suggest that long lipophilic compounds like lauryl or stearyl alcohol should be chosen for the development of the synthetic antigen convenient for direct ELISA.
言語
eng
資源タイプ text
ジャンル Departmental Bulletin Paper
Index
/ Public / 奈良大学紀要 / 16号
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